• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Quantitative Genetic Bases of Anthocyanin Variation in Grape (Vitis vinifera L. ssp. sativa) Berry: A Quantitative Trait Locus to Quantitative Trait Nucleotide Integrated Study
【2h】

Quantitative Genetic Bases of Anthocyanin Variation in Grape (Vitis vinifera L. ssp. sativa) Berry: A Quantitative Trait Locus to Quantitative Trait Nucleotide Integrated Study

机译：葡萄（Vitis vinifera L.ssp.sativa）浆果中花色苷变异的定量遗传基础：定量性状位点到定量性状核苷酸的综合研究

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The combination of QTL mapping studies of synthetic lines and association mapping studies of natural diversity represents an opportunity to throw light on the genetically based variation of quantitative traits. With the positional information provided through quantitative trait locus (QTL) mapping, which often leads to wide intervals encompassing numerous genes, it is now feasible to directly target candidate genes that are likely to be responsible for the observed variation in completely sequenced genomes and to test their effects through association genetics. This approach was performed in grape, a newly sequenced genome, to decipher the genetic architecture of anthocyanin content. Grapes may be either white or colored, ranging from the lightest pink to the darkest purple tones according to the amount of anthocyanin accumulated in the berry skin, which is a crucial trait for both wine quality and human nutrition. Although the determinism of the white phenotype has been fully identified, the genetic bases of the quantitative variation of anthocyanin content in berry skin remain unclear. A single QTL responsible for up to 62% of the variation in the anthocyanin content was mapped on a Syrah × Grenache F1 pseudo-testcross. Among the 68 unigenes identified in the grape genome within the QTL interval, a cluster of four Myb-type genes was selected on the basis of physiological evidence (VvMybA1, VvMybA2, VvMybA3, and VvMybA4). From a core collection of natural resources (141 individuals), 32 polymorphisms revealed significant association, and extended linkage disequilibrium was observed. Using a multivariate regression method, we demonstrated that five polymorphisms in VvMybA genes except VvMybA4 (one retrotransposon, three single nucleotide polymorphisms and one 2-bp insertion/deletion) accounted for 84% of the observed variation. All these polymorphisms led to either structural changes in the MYB proteins or differences in the VvMybAs promoters. We concluded that the continuous variation in anthocyanin content in grape was explained mainly by a single gene cluster of three VvMybA genes. The use of natural diversity helped to reduce one QTL to a set of five quantitative trait nucleotides and gave a clear picture of how isogenes combined their effects to shape grape color. Such analysis also illustrates how isogenes combine their effect to shape a complex quantitative trait and enables the definition of markers directly targeted for upcoming breeding programs.
机译：合成系的QTL作图研究与自然多样性的关联作图研究相结合，为阐明基于遗传的数量性状变异提供了机会。利用通过定量性状基因座（QTL）定位提供的位置信息（通常会导致包含许多基因的宽间隔），现在可以直接靶向可能对完全测序的基因组中观察到的变异负责的候选基因并进行测试它们通过关联遗传学的作用。这种方法是在葡萄（一种新测序的基因组）中进行的，以破译花色苷含量的遗传结构。葡萄可以是白色的也可以是彩色的，根据浆果皮中所积累的花青素的数量，从最浅的粉红色到最暗的紫色，这对于葡萄酒质量和人类营养都是至关重要的特征。尽管已经完全确定了白色表型的确定性，但浆果皮中花色苷含量的定量变化的遗传基础仍然不清楚。在Syrah×Grenache F1伪测试杂交板上绘制了一个单独的QTL，该QTL负责花色苷含量变化的62％。在QTL间隔内的葡萄基因组中鉴定出的68个单基因中，根据生理证据（VvMybA1，VvMybA2，VvMybA3和VvMybA4）选择了四个Myb型基因的簇。从核心资源（141个个体）的核心集合中，发现32个多态性具有明显的关联性，并且观察到扩展的连锁不平衡。使用多元回归方法，我们证明了VvMybA基因中除VvMybA4以外的五个多态性（一个反转录转座子，三个单核苷酸多态性和一个2 bp插入/缺失）占观察到的变异的84％。所有这些多态性导致MYB蛋白的结构变化或VvMybAs启动子的差异。我们得出的结论是，葡萄中花色苷含量的连续变化主要由三个VvMybA基因的单个基因簇解释。利用自然多样性有助于将一个QTL减少为一组五个定量性状核苷酸，并清楚地显示了同基因如何结合其作用来塑造葡萄的颜色。此类分析还说明了同基因如何结合其作用以形成复杂的定量性状，并能够定义直接针对即将到来的育种计划的标记。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号